{"product_id":"recombinant-mouse-cystatin-7-cst7-protein-fc-tagactive-pksm040785","title":"Recombinant Mouse Cystatin 7\/CST7 Protein (Fc Tag)(Active) - PKSM040785","description":"\u003cp\u003eRecombinant Mouse Cystatin 7\/CST7 Protein (Fc Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 20μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSM040785-20\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Cystatin 7; CST7\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Cystatin-F; Cystatin-like Metastasis-Associated Protein; Leukocystatin; CMAP; Cystatin-7; Cst7\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Enzyme\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-hFc\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: O89098\u003c\/p\u003e\n\n\u003cp\u003eAccession: O89098\u003c\/p\u003e\n\n\u003cp\u003eBackground: The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC, R\u0026amp;D Systems, Catalog # ES008.The IC50 is \u0026lt; 6 nM.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met1-Gln144\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 93 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 25mM Tris, 0.15M NaCl, pH 7.5\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 41.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 42 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104363905203,"sku":"PKSM040785-20","price":856.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_3735d13a-759a-4e56-9e73-5d5022236087.png?v=1709295254","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-cystatin-7-cst7-protein-fc-tagactive-pksm040785","provider":"AFSBio Inc.","version":"1.0","type":"link"}