{"product_id":"recombinant-mouse-egfr-protein-his-tagactive-pksm040365","title":"Recombinant Mouse EGFR Protein (His Tag)(Active) - PKSM040365","description":"Recombinant Mouse EGFR Protein (His Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSM040365-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: EGFR\n\nTarget Symptom: 9030024J15Rik; AI552599; Erbb; Errb1; Errp; wa-2; wa2; Wa5\n\nTarget Species: Mouse\n\nExpression Host: HEK293 Cells\n\nApplication: ELISA\n\nFusion Tag: C-His\n\nUNIProt ID: Q01279\n\nAccession: Q01279\n\nBackground: As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α , betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K\/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.\n\nActivity: 1. Immobilized mouse EGFR-his at 10 μg\/mL (100 μl\/well) can bind human EGF-Fc, The EC50 of human EGF-Fc is 60-90 ng\/mL. \n2. Immobilized mouse EGFR-his at 10 μg\/mL (100 μl\/well) can bind mouse EGF-Fc, The EC50 of mouse EGF-Fc is 70-100 ng\/mL. \n\nSequence: Met 1-Ser 647\n\nPurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 71 kDa\n\nObserved MW: 100 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104354631859,"sku":"PKSM040365-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_8b323c01-2f8b-45d3-a89b-9e95d4d3399c.png?v=1709295079","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-egfr-protein-his-tagactive-pksm040365","provider":"AFSBio Inc.","version":"1.0","type":"link"}