{"product_id":"recombinant-mouse-enpp7-npp-7-protein-his-tag-pksm040611","title":"Recombinant Mouse ENPP7\/NPP-7 Protein (His Tag) - PKSM040611","description":"\u003cp\u003eRecombinant Mouse ENPP7\/NPP-7 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSM040611-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: ENPP7, NPP-7\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Alk-SMase, Gm254\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q3TIW9\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q3TIW9\u003c\/p\u003e\n\n\u003cp\u003eBackground: Ectonucleotide pyrophosphatase \/ phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase \/ phosphodiesterase family. ENPP7 \/ NPP-7 is expressed in the intestines and human bile. ENPP7 \/ NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 \/ NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 \/ NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 \/ NPP-7 may be associated with human colon cancer.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Gln 421\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.6\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 47 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 60 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"50μg","offer_id":43155010683059,"sku":"PKSM040611-50","price":1213.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_6826c242-9253-4357-8a3c-d53837846af3.png?v=1710436234","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-enpp7-npp-7-protein-his-tag-pksm040611","provider":"AFSBio Inc.","version":"1.0","type":"link"}