{"product_id":"recombinant-mouse-epo-receptor-epor-protein-his-tagactive-pksm040899","title":"Recombinant Mouse EPO Receptor\/EPOR Protein (His Tag)(Active) - PKSM040899","description":"Recombinant Mouse EPO Receptor\/EPOR Protein (His Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSM040899-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: EPO Receptor; EPOR\n\nTarget Symptom: Epor\n\nTarget Species: Mouse\n\nExpression Host: HEK293 Cells\n\nApplication: Cell culture; ELISA\n\nFusion Tag: C-His\n\nAccession: NP_034279.3\n\nBackground: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR\/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â … single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.\n\nActivity: 1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 μg\/mL in the presence of 16 ng\/mL Recombinant mouse EPO. \n2. Immobilized mouse EPOR-His at 10μg\/mL (100μL\/well) can bind biotinylated mouse EPO-His. The EC50 of biotinylated mouse EPO-His is 34. 5-80.6ng\/mL. \n\nSequence: Met 1-Pro 249\n\nPurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 26.2 kDa\n\nObserved MW: 30-35 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104277725363,"sku":"PKSM040899-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_1c6022cf-89b1-4797-9635-5fdb8906381d.png?v=1709293589","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-epo-receptor-epor-protein-his-tagactive-pksm040899","provider":"AFSBio Inc.","version":"1.0","type":"link"}