{"product_id":"recombinant-mouse-fgf-9-fgf9-protein-his-tagactive-pksm041022","title":"Recombinant Mouse FGF-9\/FGF9 Protein (His Tag)(Active) - PKSM041022","description":"\u003cp\u003eRecombinant Mouse FGF-9\/FGF9 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSM041022-10, PKSM041022-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: FGF-9; FGF9\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Fibroblast growth factor 9; FGF-9; Glia-activating factor; GAF; heparin-binding growth factor-9; HBGF-9; Fgf9; Fgf-9\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P54130\u003c\/p\u003e\n\n\u003cp\u003eAccession: P54130\u003c\/p\u003e\n\n\u003cp\u003eBackground: Fibroblast growth factor-9 (FGF-9) is an approximately 26 kDa secreted glycoprotein of the FGF family. Secreted mouse FGF-9 lacks the N-terminal 1-3 aa and shares \u0026gt;98% sequence identity with rat, human, equine, porcine and bovine FGF-9. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In the mouse embryo the location and timing of FGF-9 expression affects development of the skeleton, cerebellum, lungs, heart, vasculature, digestive tract, and testes .It may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. Deletion of mouse FGF-9 is lethal at birth due to lung hypoplasia, and causes rhizomelia, or shortening of the proximal skeleton. An unusual constitutive dimerization of FGF 9 buries receptor interaction sites which lowers its activity, and increases heparin affinity which inhibits diffusion. A spontaneous mouse mutant, Eks, interferes with dimerization, resulting monomeric, diffusible FGF-9 that causes elbow and knee synostoses (joint fusions) due to FGF-9 misexpression in developing joints.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cell proliferation assay using Balb\/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 4. 14 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met1-Ser208\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 5%Trehalose, 1mM EDTA, 20%glycerol, 1mM DTT, pH8.5 .\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 24.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 25 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43114030891187,"sku":"PKSM041022-10","price":385.0,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43114030923955,"sku":"PKSM041022-50","price":735.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_93c7a5f9-46b2-4651-833c-a0154f72856f.png?v=1709550528","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-fgf-9-fgf9-protein-his-tagactive-pksm041022","provider":"AFSBio Inc.","version":"1.0","type":"link"}