{"product_id":"recombinant-mouse-igf-ii-proteinn-hisactive-pksm041521","title":"Recombinant Mouse IGF-II protein(N-His)(active) - PKSM041521","description":"Recombinant Mouse IGF-II protein(N-His)(active)\n\nSizes: 20μg, 100μg\n\nCatalogue Numbers: PKSM041521-20, PKSM041521-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: IGF-II\n\nTarget Symptom: AI429687\n\nTarget Species: Mouse\n\nExpression Host: E.coli\n\nApplication: Cell culture\n\nFusion Tag: N-His\n\nUNIProt ID: P09535\n\nAccession: P09535\n\nBackground: Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine and porcine proteins) and exhibit cross-species activity.\nIGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.\n\nActivity: Measure by its ability to induce MCF-7 cells proliferation. The ED50 for this effect is \u0026lt; 6 ng\/mL. The specific activity of recombinant mouse IGF-II is \u0026gt; 1. 5 x 10\u003csup\u003e5\u003c\/sup\u003eIU\/mg.\n\nSequence: MGIPVGKSMLVLLISLAFALCCIAAYGPGETLCGGELVDTLQFVCSDRGFYFSRPSSRANRRSRGIVEECCFRSCDLALLETYCATPAKSERDVSTSQAVLPDDFPRYPVGKFFQYDTWRQSAGRLRRGLPALLRARRGRMLAKELKEFREAKRHRPLIVLPPKDPAHGGASSEMSSNHQ\n\nPurity: \u0026gt; 98 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 8.0\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 0.1 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 20.86 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43118186528947,"sku":"PKSM041521-20","price":348.6,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43118186561715,"sku":"PKSM041521-100","price":1034.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_6fdaffcf-c209-4218-84cd-6cceae7f329f.png?v=1709637741","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-igf-ii-proteinn-hisactive-pksm041521","provider":"AFSBio Inc.","version":"1.0","type":"link"}