{"product_id":"recombinant-mouse-il-1-beta-protein-rp01340","title":"Recombinant Mouse IL-1 beta Protein - RP01340","description":"\u003cp\u003eRecombinant Mouse IL-1 beta Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01340-10, RP01340-20, RP01340-50, RP01340-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Mouse IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val118-Ser269) of mouse IL-1 beta (Accession #NP_032387.1) fused with 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IL-1 beta, IL-, Il-1b, IL-1beta, Il1b\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P10749\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 16176\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1\/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2\/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse IL1B at 10 μg\/mL (100 μL\/well) can bind Human IL1R2 with a linear range of 0.03125-0.8036 μg\/mL.|2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 42.1-168.4 pg\/mL, corresponding to a specific activity of 5.94×10^6 -2.37×10^7units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: E. coli\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: VPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVAL GLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNW YISTSQAEHKPVFLGNNSGQDIIDFTMESVSS\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198344814771,"sku":"RP01340-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198344847539,"sku":"RP01340-20","price":308.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198344880307,"sku":"RP01340-50","price":490.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198344913075,"sku":"RP01340-100","price":784.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_8c885baa-ce4e-4df0-8c7a-b6382e5d08cf.png?v=1766960849","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-il-1-beta-protein-rp01340","provider":"AFSBio Inc.","version":"1.0","type":"link"}