{"product_id":"recombinant-mouse-il-12-il-12a-il-12b-protein-rp01691","title":"Recombinant Mouse IL-12\/IL-12A\u0026IL-12B Protein - RP01691","description":"\u003cp\u003eRecombinant Mouse IL-12\/IL-12A\u0026amp;IL-12B Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01691-10, RP01691-20, RP01691-50, RP01691-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Active Recombinant Mouse IL-12\/IL-12A\u0026amp;IL-12B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg23-Ala215＆Met23-Ser335) of Mouse IL-12A\u0026amp;IL-12B (Accession #P43431＆P43432) fused with and a 6×His tag at the C-terminus(IL-12).\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: p35; IL12; Il-12a; IL-12p35\u0026amp;p40; Il-12b; Il12p40; Il-12p40, IL-12\u0026amp;Il12b; IL-12 p70 (IL12A\u0026amp;IL12B)\u003c\/p\u003e \n\n\u003cp\u003eSWISS: P43431＆P43432\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 16159\u0026amp;16160\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE; ≥ 95 % as determined by HPLC.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Measured by its binding ability in a functional ELISA.Immobilized Mouse IL-12A\u0026amp;IL-12B (Catalog: RP01691) at 5 μg\/mL (100 μL\/well) can bind Human IL12RB1 with a linear range of 0.001-3.3 ng\/mL.|2.Measured in a cell proliferation assay using IL2-activated mouse splenocytes. The ED50 for this effect is 0.01- 0.03 ng\/mL, corresponding to a specific activity of 3.3×10^7~ 1× 10 ^8 units\/mg.|3.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 7.3-29.2 ng\/mL, corresponding to a specific activity of 3.4×10^4 ~1.4×10^5 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His(IL-12a)\u0026amp;No tag(IL-12b)\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: RVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA\u0026amp;MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198339113139,"sku":"RP01691-10","price":168.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198339145907,"sku":"RP01691-20","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198339178675,"sku":"RP01691-50","price":420.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198339211443,"sku":"RP01691-100","price":672.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_5503c8b2-77b0-46bf-9680-727c843813ce.png?v=1766960778","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-il-12-il-12a-il-12b-protein-rp01691","provider":"AFSBio Inc.","version":"1.0","type":"link"}