{"product_id":"recombinant-mouse-il-17a-ctla-8-protein-rp01460","title":"Recombinant Mouse IL-17A\/CTLA-8 Protein - RP01460","description":"\u003cp\u003eRecombinant Mouse IL-17A\/CTLA-8 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01460-10, RP01460-20, RP01460-50, RP01460-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Mouse IL-17A\/CTLA-8 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala26-Ala158) of mouse IL-17A\/CTLA-8 (Accession #NP_034682.1.) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Il17, Ctla8, IL-17, Ctla-8, IL17A, IL-17A\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q62386-1\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 16171\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 90 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival\/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17\/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E\/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2\/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-17A\/CTLA-8 at 2 μg\/mL (100 μL\/well) can bind Mouse IL-17RA\/CD217 with a linear range of 0.1-26 ng\/mL.Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 2 ng\/mL TNFα(Catalog: RP01071).The ED50 for this effect is 0.26-1.04 ng\/mL, corresponding to a specific activity of 9.62×10^5 ~3.85×10^6 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: AAIIPQSSACPNTEAKDFLQNVKVNLKVFNSLGAKVSSRRPSDYLNRSTSPWTLHRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILVLKREPESCPFTFRVEKMLVGVGCTCVASIVRQAA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198310604979,"sku":"RP01460-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198310637747,"sku":"RP01460-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198310670515,"sku":"RP01460-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198310703283,"sku":"RP01460-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_a895d8ad-3dbf-4c00-854b-ee6854a0b8f5.png?v=1766960453","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-il-17a-ctla-8-protein-rp01460","provider":"AFSBio Inc.","version":"1.0","type":"link"}