{"product_id":"recombinant-mouse-il-23-il-12b-il-23a-protein-rp02928s","title":"Recombinant Mouse IL-23\/IL-12B\u0026IL-23A Protein - RP02928S","description":"\u003cp\u003eRecombinant Mouse IL-23\/IL-12B\u0026amp;IL-23A Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP02928S-10, RP02928S-20, RP02928S-50, RP02928S-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Mouse IL-23\/IL-12B\u0026amp;IL-23A Protein is produced by HEK293 expression system. The target protein is expressed with sequence (N-His(IL-23a)\u0026amp;No tag(IL-12b)) of mouse Met23-Ser335＆Ala21-Ala196 (Accession #) fused with a 6×His tag at the N-terminus(IL23A).\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: p40; Il-12b; Il12p40; Il-12p40; IL12B; p19; IL-23; il23A\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P43432＆Q9EQ14\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 16160\u0026amp;83430\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 90% as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T?cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T?cells. While IL-12 acts on both na?ve and memory human Tnbsp; cells, the effects of IL-23 is restricted to memory Tcells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor\/self peptide.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 29.1-116.4 pg\/mL, corresponding to a specific activity of 8.59×10^6 ~3.44×10^7 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: N-His(IL-23a)\u0026amp;No tag(IL-12b)\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS\u0026amp;AVPRSSSPDWAQCQQLSRNLCMLAWNAHAPAGHMNLLREEEDEETKNNVPRIQCEDGCDPQGLKDNSQFCLQRIRQGLAFYKHLLDSDIFKGEPALLPDSPMEQLHTSLLGLSQLLQPEDHPRETQQMPSLSSSQQWQRPLLRSKILRSLQAFLAIAARVFAHGAATLTEPLVPTA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198328725683,"sku":"RP02928S-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198328758451,"sku":"RP02928S-20","price":308.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198328791219,"sku":"RP02928S-50","price":490.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198328823987,"sku":"RP02928S-100","price":784.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_6b16d9f0-3414-4488-b2b7-c7017a4e6209.png?v=1766960648","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-il-23-il-12b-il-23a-protein-rp02928s","provider":"AFSBio Inc.","version":"1.0","type":"link"}