{"product_id":"recombinant-mouse-il-25-il-17e-protein-rp01799","title":"Recombinant Mouse IL-25\/IL-17E Protein - RP01799","description":"\u003cp\u003eRecombinant Mouse IL-25\/IL-17E Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01799-10, RP01799-20, RP01799-50, RP01799-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Mouse IL-25\/IL-17E Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val 17-Ala 169) of mouse IL17E (Accession #NP_542767) fused with and a 6×His tag at the N-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IL-25; Il17e; IL-17e, IL25\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q8VHH8\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 140806\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 90 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)–activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its ability to induce CXCL1\/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 0.96-3.83 ng\/mL, corresponding to a specific activity of 2.61×10^5 ~1.04×10^6 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: N-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: VSLRIQEGCSHLPSCCPSKEQEPPEEWLKWSSASVSPPEPLSHTHHAESCRASKDGPLNSRAISPWSYELDRDLNRVPQDLYHARCLCPHCVSLQTGSHMDPLGNSVPLYHNQTVFYRRPCHGEEGTHRRYCLERRLYRVSLACVCVRPRVMA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.01 EU\/μg of the protein by LAL method\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198340161715,"sku":"RP01799-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198340194483,"sku":"RP01799-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198340227251,"sku":"RP01799-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198340260019,"sku":"RP01799-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_454a1010-6a5e-44c9-b90e-fd1e7705e3f5.png?v=1766960790","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-il-25-il-17e-protein-rp01799","provider":"AFSBio Inc.","version":"1.0","type":"link"}