{"product_id":"recombinant-mouse-interleukin-13-il-13-protein-aa-26-131-his-tagactive-pksm041074","title":"Recombinant Mouse Interleukin-13\/IL-13 Protein (aa 26-131,His Tag)(Active) - PKSM041074","description":"\u003cp\u003eRecombinant Mouse Interleukin-13\/IL-13 Protein (aa 26-131,His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSM041074-10, PKSM041074-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Interleukin-13; IL-13\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Interleukin-13; IL-13; T-Cell Activation Protein P600; Il13; Il-13\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P20109\u003c\/p\u003e\n\n\u003cp\u003eAccession: P20109\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-13 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ser26-Phe131\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 12.7 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 15-30 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43114034561203,"sku":"PKSM041074-10","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43114034593971,"sku":"PKSM041074-50","price":732.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_1e348866-0110-4082-8d1f-1e80e437a22a.png?v=1709550567","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-interleukin-13-il-13-protein-aa-26-131-his-tagactive-pksm041074","provider":"AFSBio Inc.","version":"1.0","type":"link"}