{"product_id":"recombinant-mouse-interleukin-18-il-18-protein-his-tag-pksm041079","title":"Recombinant Mouse Interleukin-18\/IL-18 Protein (His Tag) - PKSM041079","description":"\u003cp\u003eRecombinant Mouse Interleukin-18\/IL-18 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSM041079-20, PKSM041079-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Interleukin-18; IL-18\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Interleukin-18; Il18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; Igif\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P70380\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P70380\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin-18 (IL-18）is a protein which belongs to the IL-1 family. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. It augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Asn36-Ser192\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 19.7 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 18 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"20μg","offer_id":43072198246579,"sku":"PKSM041079-20","price":348.6,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43072198279347,"sku":"PKSM041079-100","price":1034.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_dcabdccd-4c62-434e-9940-f8c1e2886b69.png?v=1708683733","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-interleukin-18-il-18-protein-his-tag-pksm041079","provider":"AFSBio Inc.","version":"1.0","type":"link"}