{"product_id":"recombinant-mouse-macrophage-migration-inhibitory-factor-mif-protein-rp01829","title":"Recombinant Mouse Macrophage migration inhibitory factor\/MIF Protein - RP01829","description":"\u003cp\u003eRecombinant Mouse Macrophage migration inhibitory factor\/MIF Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01829-10, RP01829-20, RP01829-50, RP01829-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Mouse Macrophage migration inhibitory factor\/MIF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro2-Ala115) of mouse Macrophage migration inhibitory factor\/MIF (Accession #NP_034928.1)\u003c\/p\u003e \n\n\u003cp\u003eAlternate Names: GIF; DER6; Glif; Macrophage migration inhibitory factor; MIF\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P34884\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 17319\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: MIF (or macrophage migration inhibitory factor) was the first lymphokine\/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4 - 7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50 - 65 have also been suggested to contain thiol-protein oxidoreductase activity (12).\u003c\/p\u003e\n\n\u003cp\u003eSource: E. coli\u003c\/p\u003e\n\n\u003cp\u003eTag: NO-Tag\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of 50mM Tris 150mM NaCl pH8.0.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: PMFIVNTNVPRASVPEGFLSELTQQLAQATGKPAQYIAVHVVPDQLMTFSGTNDPCALCSLHSIGKIGGAQNRNYSKLLCGLLSDRLHISPDRVYINYYDMNAANVGWNGSTFA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198268465331,"sku":"RP01829-10","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198268498099,"sku":"RP01829-20","price":266.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198268530867,"sku":"RP01829-50","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198268563635,"sku":"RP01829-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_bd9773e6-8007-4097-adac-806a812f4d52.png?v=1766959928","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-macrophage-migration-inhibitory-factor-mif-protein-rp01829","provider":"AFSBio Inc.","version":"1.0","type":"link"}