{"product_id":"recombinant-mouse-pd-l1-b7-h1-cd274-protein-his-tag-active-pksm040919","title":"Recombinant Mouse PD-L1\/B7-H1\/CD274 Protein (His Tag) (Active) - PKSM040919","description":"\u003cp\u003eRecombinant Mouse PD-L1\/B7-H1\/CD274 Protein (His Tag) (Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSM040919-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PD-L1; B7-H1; CD274\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: A530045L16Rik Protein; Mouse; B7h1 Protein; Mouse; Pdcd1l1 Protein; Mouse; Pdcd1lg1 Protein; Mouse; Pdl1 Protein; Mouse\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_068693.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1\/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1\/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28\/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized recombinant mouse PD1-L1 at 1 μg\/ml (100 μl\/well) can bind mouse PD1 with a linear range of 6. 25-400 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met 1-Thr 238\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 98 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 26.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 40-45 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104278511795,"sku":"PKSM040919-100","price":938.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_a9b2ebf8-0812-4a0f-87aa-cfe7d4880b3d.png?v=1709293608","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-pd-l1-b7-h1-cd274-protein-his-tag-active-pksm040919","provider":"AFSBio Inc.","version":"1.0","type":"link"}