{"product_id":"recombinant-mouse-pdk4-protein-his-gst-tagactive-pksm040290","title":"Recombinant Mouse PDK4 Protein (His \u0026 GST Tag)(Active) - PKSM040290","description":"\u003cp\u003eRecombinant Mouse PDK4 Protein (His \u0026amp; GST Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSM040290-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PDK4\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: AV005916\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His-GST\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: O70571\u003c\/p\u003e\n\n\u003cp\u003eAccession: O70571\u003c\/p\u003e\n\n\u003cp\u003eBackground: Pyruvate dehydrogenase kinase 4 (PDK4) is a mitochondrial protein that regulates the TCA cycle.PDK4, a vital mitochondrial protein, controls the switch between glycolysis and oxidative phosphorylation based upon nutrient availability.Pyruvate dehydrogenase kinase 4 (PDK4) mRNA has been reported as an up-regulated gene in the heart and skeletal muscle of carnitine-deficient juvenile visceral steatosis (JVS) mice under fed conditions. PDK4 plays an important role in the inhibition of glucose oxidation via the phosphorylation of pyruvate dehydrogenase complex (PDC).PDK4 gene expression is stimulated by thyroid hormone (T(3)), glucocorticoids, and long chain fatty acids.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Kinase activity untested\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met1-Val412\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 85 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0 \u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 74.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 65 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104333070515,"sku":"PKSM040290-50","price":1003.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_2c36b7b5-2446-4a9f-87e0-e12c90d1855f.png?v=1709294546","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-pdk4-protein-his-gst-tagactive-pksm040290","provider":"AFSBio Inc.","version":"1.0","type":"link"}