{"product_id":"recombinant-mouse-smad2-protein-his-gst-tag-pksm040517","title":"Recombinant Mouse SMAD2 Protein (His \u0026 GST Tag) - PKSM040517","description":"\u003cp\u003eRecombinant Mouse SMAD2 Protein (His \u0026amp; GST Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSM040517-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: SMAD2\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: 7120426M23Rik, Madh2, Madr2, mMad2, Smad-2\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q62432\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His-GST\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q62432\u003c\/p\u003e\n\n\u003cp\u003eBackground: SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta\/activin\/BMP-2\/4 cytokine superfamily from receptor Ser\/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ser2-Ser467\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 8.0, 10% glycerol, 3mM DTT\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 80 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 90 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43155009142963,"sku":"PKSM040517-100","price":1197.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_a2061338-a88b-4947-80b8-b8bb99402e3d.png?v=1710436161","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-smad2-protein-his-gst-tag-pksm040517","provider":"AFSBio Inc.","version":"1.0","type":"link"}