{"product_id":"recombinant-mouse-smad5-protein-his-gst-tag-pksm040516","title":"Recombinant Mouse Smad5 Protein (His \u0026 GST Tag) - PKSM040516","description":"\u003cp\u003eRecombinant Mouse Smad5 Protein (His \u0026amp; GST Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSM040516-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Smad5\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: 1110051M15Rik, AI451355, Dwf-C, Madh5, MusMLP\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P97454\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His-GST\u003c\/p\u003e\n\n\u003cp\u003eAccession: P97454\u003c\/p\u003e\n\n\u003cp\u003eBackground: SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta\/activin\/BMP-2\/4 cytokine superfamily from receptor Ser\/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Thr2-Ser465\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 79.9 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 75-88 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43155009077427,"sku":"PKSM040516-100","price":1197.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_604f0f4b-67d5-4929-b9e3-0bb6ece895ec.png?v=1710436158","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-smad5-protein-his-gst-tag-pksm040516","provider":"AFSBio Inc.","version":"1.0","type":"link"}