{"product_id":"recombinant-mouse-tigit-protein-rp01516lq","title":"Recombinant Mouse TIGIT Protein - RP01516LQ","description":"\u003cp\u003eRecombinant Mouse TIGIT Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01516LQ-10, RP01516LQ-20, RP01516LQ-50, RP01516LQ-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Human TIGIT Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly26-Gly141) of human TIGIT (Accession #NP_001139797.1) fused with a mFc tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: TIGIT, VSIG9, VSTM3, TIGIT, TIGIT, VSIG9, VSTM3, TIGIT\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P86176\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 100043314\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 90% as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eBackground: TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an \"alternative self\" mechanism for MHC class I inhibition.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse CD155 at 2 μg\/mL (100 μL\/well) can bind Mouse TIGIT with a linear range of 2-9 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-mFc\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: GTIDTKRNISAEEGGSVILQCHFSSDTAEVTQVDWKQQDQLLAIYSVDLGWHVASVFSDRVVPGPSLGLTFQSLTMNDTGEYFCTYHTYPGGIYKGRIFLKVQESSVAQFQTAPLG\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198328987827,"sku":"RP01516LQ-10","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198329020595,"sku":"RP01516LQ-20","price":266.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198329053363,"sku":"RP01516LQ-50","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198329086131,"sku":"RP01516LQ-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_e685863d-e598-40e1-b12e-329c5657b1c2.png?v=1766960651","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-tigit-protein-rp01516lq","provider":"AFSBio Inc.","version":"1.0","type":"link"}