{"product_id":"recombinant-mouse-tnf-alpha-protein-rp01071","title":"Recombinant Mouse TNF-alpha Protein - RP01071","description":"\u003cp\u003eRecombinant Mouse TNF-alpha Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01071-10, RP01071-20, RP01071-50, RP01071-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Mouse TNF-alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu80-Leu235) of mouse TNF-alpha (Accession #NP_038721.1) fused with a 6×His tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: DIF, Tnfa, TNF-a, TNFSF2, Tnlg1f, Tnfsf1a, TNFalpha, TNF-alpha, TNF\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P06804\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 21926\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE; ≥95 % as determined by HPLC.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: 1.Immobilized recombinant Mouse TNFα at 2 μg\/mL (100 μL\/well) can bind Human TNFRSF1A with a linear range of 4-53 ng\/mL.│2.Immobilized Recombinant Mouse TNF-α at 2 μg\/mL (100 μL\/well) can bind Mouse TNFRSF1B with a linear range of 0.02-0.77 ng\/mL.│3.Recombinant Mouse TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3.55-14.2 pg\/mL, corresponding to a specific activity of 7.04×107~2.82×108 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198288224435,"sku":"RP01071-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198288257203,"sku":"RP01071-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198288289971,"sku":"RP01071-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198288322739,"sku":"RP01071-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_f8e5aa64-9848-4c94-855e-f4bc41bc871d.png?v=1766960166","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-tnf-alpha-protein-rp01071","provider":"AFSBio Inc.","version":"1.0","type":"link"}