{"product_id":"recombinant-mouse-tnfsf5-cd40-ligand-cd154-protein-rp01734","title":"Recombinant Mouse TNFSF5\/CD40 ligand\/CD154 Protein - RP01734","description":"\u003cp\u003eRecombinant Mouse TNFSF5\/CD40 ligand\/CD154 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 20g, 50ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RP01734-10, RP01734-20, RP01734-50, RP01734-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant Mouse TNFSF5\/CD40 ligand\/CD154 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly115-Leu260) of mouse CD40-L\/CD40L\/TNFSF5\/TRAP\/CD40LG (Accession #NP_035746.2) fused with and a 6×His tag at the N-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b; CD40LG\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P27548\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 21947\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Mouse\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its binding ability in a functional ELISA.Immobilized Mouse CD40L (Catalog: RP01734) at 5 μg\/mL (100 μL\/well) can bind Human CD40 (Catalog: RP01218) with a linear range of 0.005-1 μg\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: N-6His\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: GDEDPQIAAHVVSEANSNAASVLQWAKKGYYTMKSNLVMLENGKQLTVKREGLYYVYTQVTFCSNREPSSQRPFIVGLWLKPSSGSERILLKAANTHSSSQLCEQQSVHLGGVFELQAGASVFVNVTEASQVIHRVGFSSFGLLKL\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198313619635,"sku":"RP01734-10","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"20ug","offer_id":45198313652403,"sku":"RP01734-20","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"50ug","offer_id":45198313685171,"sku":"RP01734-50","price":350.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198313717939,"sku":"RP01734-100","price":560.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_00d82d9e-df06-40a9-9e8e-62928ddfb145.png?v=1766960486","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-tnfsf5-cd40-ligand-cd154-protein-rp01734","provider":"AFSBio Inc.","version":"1.0","type":"link"}