{"product_id":"recombinant-mouse-vcam1-protein-his-fc-tagactive-pksm040832","title":"Recombinant Mouse VCAM1 Protein (His \u0026 Fc Tag)(Active) - PKSM040832","description":"Recombinant Mouse VCAM1 Protein (His \u0026amp; Fc Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSM040832-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: VCAM1\n\nTarget Symptom: CD106; Vascular cell adhesion protein 1; Vcam1; L1CAM; VCMA1; Vcam-1\n\nTarget Species: Mouse\n\nExpression Host: HEK293 Cells\n\nApplication: Cell culture; ELISA\n\nFusion Tag: C-His-Fc\n\nAccession: NP_035823.3\n\nBackground: Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α 4ß 7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an \" endothelial cell gate\" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.\n\nActivity: Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1-coated plates (10 μg\/ml, 100 μg\/well), approximately \u0026gt; 70% cells will adhere specifically.\n\nSequence: Met 1-Glu 698\n\nPurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 102 kDa\n\nObserved MW: 110-120 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104273105075,"sku":"PKSM040832-100","price":680.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_e9712702-85da-49ad-bdaf-66009827e34b.png?v=1709293540","url":"https:\/\/afsbio.com\/products\/recombinant-mouse-vcam1-protein-his-fc-tagactive-pksm040832","provider":"AFSBio Inc.","version":"1.0","type":"link"}