{"product_id":"recombinant-murine-granulocyte-macrophage-colony-stimulating-factor-rmugm-csf-pr2017","title":"Recombinant Murine Granulocyte- Macrophage Colony Stimulating Factor (rMuGM-CSF) - PR2017","description":"\u003cp\u003eRecombinant Murine Granulocyte- Macrophage Colony Stimulating Factor (rMuGM-CSF)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR2017-2, PR2017-10\u003c\/p\u003e\n\n\u003cp\u003eSizes: 2µg, 10µg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: Recombinant murine GM-CSF is a 14.2 kDa globular protein consisting of 124 amino acids residues.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;95% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is ≤ 0.2 ng\/ml, corresponding to a specific activity of ≥ 5 x 106 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MAPTRSPIt V TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 1EU\/mg of rmGM-CSF as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China\u003c\/p\u003e\n\n\u003cp\u003eDescription: GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes\/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3 - 5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled α-helices (8 - 10). Mature mouse GM-CSF shares 49% - 54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF Rα\/CD116 and the signal transducing common β chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF Rα (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"2µg","offer_id":42882229502131,"sku":"PR2017-2","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"10µg","offer_id":42882229534899,"sku":"PR2017-10","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_5d7ae27d-f756-4bf5-b97b-ea44783ff55e.jpg?v=1702049693","url":"https:\/\/afsbio.com\/products\/recombinant-murine-granulocyte-macrophage-colony-stimulating-factor-rmugm-csf-pr2017","provider":"AFSBio Inc.","version":"1.0","type":"link"}