{"product_id":"recombinant-murine-stromal-cell-derived-factor-1-alpha-cxcl12-rmusdf-1a-cxcl12-pr2035","title":"Recombinant Murine Stromal-Cell Derived Factor-1 alpha\/CXCL12 (rMuSDF-1a\/CXCL12) - PR2035","description":"\u003cp\u003eRecombinant Murine Stromal-Cell Derived Factor-1 alpha\/CXCL12 (rMuSDF-1a\/CXCL12)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR2035-2, PR2035-10\u003c\/p\u003e\n\n\u003cp\u003eSizes: 2µg, 10µg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;95% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Measured by its ability to chemoattract human CXCR4 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 0.15-0.6 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 1EU\/μg of rMuSDF-1a\/CXCL12 as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China\u003c\/p\u003e\n\n\u003cp\u003eDescription: SDF-1α and SDF-1β, members of the chemokine α subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1α and SDF-1β cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1α and SDF-1β are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1β and absent from SDF-1α. SDF-1\/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1\/PBSF acts via the chemokine receptor CXCR4 and has been shown to be a chemoattractant for T-lymphocytes, monocytes, pro- and pre- B cells, but not neutrophils. Mice lacking SDF-1 or CXCR4 have been found to have impaired B-lymphopoiesis, myelopoiesis, vascular development, cardiogenesis and abnormal neuronal cell migration and patterning in the central nervous system .\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"2µg","offer_id":42882222620851,"sku":"PR2035-2","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"10µg","offer_id":42882222653619,"sku":"PR2035-10","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b9765d4c-9ec5-463a-bdbf-36f3a36015da.jpg?v=1702049544","url":"https:\/\/afsbio.com\/products\/recombinant-murine-stromal-cell-derived-factor-1-alpha-cxcl12-rmusdf-1a-cxcl12-pr2035","provider":"AFSBio Inc.","version":"1.0","type":"link"}