{"product_id":"recombinant-murine-tumor-necrosis-factor-alpha-rmutnf-α-pr2036","title":"Recombinant Murine Tumor Necrosis Factor-alpha (rMuTNF-α) - PR2036","description":"\u003cp\u003eRecombinant Murine Tumor Necrosis Factor-alpha (rMuTNF-α)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR2036-5, PR2036-20\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5µg, 20µg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: Approximately 17.3 kDa. The recombinant murine TNF-alpha is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;97% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is \u0026lt; 0.1 ng\/ml, corresponding to a specific activity of \u0026gt; 1×107 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.2.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVY SQVLFKGQGC PDYVLLTHt V SRFAISYQEKVNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 1EU\/mg of rMuTNF-α as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China\u003c\/p\u003e\n\n\u003cp\u003eDescription: Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-α occurs as a membrane-anchored form. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"5µg","offer_id":42882230190259,"sku":"PR2036-5","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"20µg","offer_id":42882230223027,"sku":"PR2036-20","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_fa735c90-74c5-4ead-aa5c-a85c58c449c2.jpg?v=1702049713","url":"https:\/\/afsbio.com\/products\/recombinant-murine-tumor-necrosis-factor-alpha-rmutnf-%ce%b1-pr2036","provider":"AFSBio Inc.","version":"1.0","type":"link"}