{"product_id":"recombinant-nt-4-human-bk0144","title":"Recombinant NT-4, Human - BK0144","description":"\u003cp\u003eRecombinant NT-4, Human\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0144-10, BK0144-50\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 28.1 kDa, a noncovalently linked homodimer,of two 14.0 kDa polypeptide monomers.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: ED50 \u0026lt; 5.0 μg\/ml, measured by a cell proliferation assay using C6 cells, corresponding to a specific activity of \u0026gt; 2.0× 10ˆ2 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against 50mM acetic acid.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MGVSETAPA SRRGELAVCD AVSGWVTDRR TAVDLRGREV EVLGEVPAAG GSPLRQYFFE TRCKADNAEE GGPGAGGGGC RGVDRRHWVS ECKAKQSYVR ALTADAQGRV GWRWIRIDTA CVCTLLSRTG RA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.3 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in 50mM acetic acid or ddH2O at 50 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant human Neurotrophin-4 (rhNT-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhNT-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Neurotrophin-4 (NT-4), also known as NT-5, is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human β-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons.Recombinant human Neurotrophin-4 (rhNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"10μg","offer_id":42882245525683,"sku":"BK0144-10","price":375.54,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":42882245558451,"sku":"BK0144-50","price":1152.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_96be79e3-4c65-4178-93fb-5d6e7394579c.jpg?v=1702049980","url":"https:\/\/afsbio.com\/products\/recombinant-nt-4-human-bk0144","provider":"AFSBio Inc.","version":"1.0","type":"link"}