{"product_id":"recombinant-nt-4-mouse-bk0145","title":"Recombinant NT-4, Mouse - BK0145","description":"\u003cp\u003eRecombinant NT-4, Mouse\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0145-10, BK0145-50\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 14.0 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% by SDS-PAGEanalysis.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: ED50 \u0026lt;1µg\/mL, measured by a cell proliferation assay usingC6cells, corresponding to a specific activity of\u0026gt;1× 10ˆ3 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against 50mM acetic acid.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MGVSETAPA SRRGELAVCD AVSGWVTDRR TAVDLRGREV EVLGEVPAAG GSPLRQYFFE TRCKAESAGE GGPGVGGGGC RGVDRRHWLS ECKAKQSYVR ALTADSQGRV GWRWIRIDTA CVCTLLSRTG RA\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in 50mM acetic acid or ddH2O at 100 μg\/mL.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant mouse Neurotrophin-4 (rmNT-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmNT-4 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Neurotrophin-4 (NT-4) is a small secreted cytokine, and belongs to the Neurotrophin (NT) family, which also includes Brain Derived Neurotropic Factor (BDNF), Nerve Growth Factor (NGF), and NT-3. NT family members are all derived from similar sized protein precursors, composed of N-terminal propeptides and C-terminal mature domains, which are separated by posttranslational proteolytic cleavage. NT-4 (along with NT-3) is foundin the brains of mammals . In vivo, NT-4 binds to the common receptor, p75NTR, and a tyrosine kinase receptor, TrkB. The heterotrimeric complex activates the NFκB transcription factor. NT-4 is essential for the differentiation and wiring regulation of the central and peripheral nervous systems during development, and is related to important diseases including Alzheimer’s.Recombinant mouse Neurotrophin-4 (rmNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chainsof 131 amino acids. A fully biologically active molecule, rmNT-4 has a molecular mass of 14.0kDaanalyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"10μg","offer_id":42882245591219,"sku":"BK0145-10","price":375.54,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":42882245623987,"sku":"BK0145-50","price":1152.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d6dc3b90-dbcb-406b-8c98-c4caf7b8475f.jpg?v=1702049982","url":"https:\/\/afsbio.com\/products\/recombinant-nt-4-mouse-bk0145","provider":"AFSBio Inc.","version":"1.0","type":"link"}