{"product_id":"recombinant-pdgf-bb-rat-bk0153","title":"Recombinant PDGF-BB, Rat - BK0153","description":"\u003cp\u003eRecombinant PDGF-BB, Rat\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0153-10, BK0153-50\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 24.6 kDa, observed by non-reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% by SDS-PAGE analysis.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: ED50 \u0026lt; 2 ng\/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of \u0026gt; 5 × 10ˆ5 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against 20 mM acetic acid.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MSLGSLAAA EPAVIAECKT RTEVFQISRN LIDRTNANFL VWPPCVEVQR CSGCCNNRNV QCRASQVQMR PVQVRKIEIV RKKPVFKKAT VTLEDHLACK CEt VVTPRPV T\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in 20 mM acetic acid at 100 μg\/mL.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrPDGF-BB remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rrPDGF-BB has a molecular mass of 24.6 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"10μg","offer_id":42882246213811,"sku":"BK0153-10","price":256.94,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":42882246246579,"sku":"BK0153-50","price":813.32,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_892de531-c85c-404e-b20c-43bf65948834.jpg?v=1702049995","url":"https:\/\/afsbio.com\/products\/recombinant-pdgf-bb-rat-bk0153","provider":"AFSBio Inc.","version":"1.0","type":"link"}