{"product_id":"recombinant-rat-cd73-nt5e-protein-his-tag-pksr030462","title":"Recombinant Rat CD73\/NT5E Protein (His Tag) - PKSR030462","description":"\u003cp\u003eRecombinant Rat CD73\/NT5E Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSR030462-10, PKSR030462-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD73; NT5E\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: CD73; E5NT; Nucleotidase; NT5E; ecto-5'-nucleotidase; 5'-Nucleotidase\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P21588\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Rat\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P21588\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73 converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Trp29-Lys549\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 59 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 70-90 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43072253886643,"sku":"PKSR030462-10","price":359.8,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43072253919411,"sku":"PKSR030462-50","price":625.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_6520459b-7b4b-4563-b1b2-5a0f1929f38b.png?v=1708685194","url":"https:\/\/afsbio.com\/products\/recombinant-rat-cd73-nt5e-protein-his-tag-pksr030462","provider":"AFSBio Inc.","version":"1.0","type":"link"}