{"product_id":"recombinant-rat-il1r1-cd121a-protein-his-fc-tagactive-pksr030395","title":"Recombinant Rat IL1R1\/CD121a Protein (His \u0026 Fc Tag)(Active) - PKSR030395","description":"Recombinant Rat IL1R1\/CD121a Protein (His \u0026amp; Fc Tag)(Active)\n\nSize: 100μg\n\nCatalogue Number: PKSR030395-100\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: IL1R1; CD121a\n\nTarget Symptom: Il1r1\n\nTarget Species: Rat\n\nExpression Host: HEK293 Cells\n\nApplication: Cell culture\n\nFusion Tag: C-His-Fc\n\nAccession: NP_037255.3\n\nBackground: Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1\/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1\/IL1RA). IL-1R1\/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1\/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1\/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.\n\nActivity: Measured by its ability to inhibit IL1α-dependent proliferation in D10.G4. 1 mouse helper T cells（Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. ). The ED50 for this effect is typically 5-20 μg\/ml in the presence of 40 pg\/mL of recombinant human IL1a.\n\nSequence: Met 1-Lys 352\n\nPurity: \u0026gt; 85 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from sterile PBS, pH 7.4\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 64.6 kDa\n\nObserved MW: 90-95 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104282443955,"sku":"PKSR030395-100","price":1024.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_2631b006-6371-42d3-8af9-cf3e68c40140.png?v=1709293687","url":"https:\/\/afsbio.com\/products\/recombinant-rat-il1r1-cd121a-protein-his-fc-tagactive-pksr030395","provider":"AFSBio Inc.","version":"1.0","type":"link"}