{"product_id":"recombinant-rat-interleukin-21-rrtil-21-pr3010","title":"Recombinant Rat Interleukin 21 (rRtIL-21) - PR3010","description":"\u003cp\u003eRecombinant Rat Interleukin 21 (rRtIL-21)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR3010-2, PR3010-10\u003c\/p\u003e\n\n\u003cp\u003eSizes: 2µg, 10µg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;95% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Measured by its ability to inhibit IL21dependent proliferation of N1186 human T cells.\u003cbr\u003e\n The ED50 for this effect is typically 1-5μg\/mL in the presence of 50 ng\/mL of recombinant mouse IL21.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE \u003cbr\u003e\n AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 1EU\/μg of rRtIL-21 as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China\u003c\/p\u003e\n\n\u003cp\u003eDescription: IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21\/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"2µg","offer_id":42882222948531,"sku":"PR3010-2","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"10µg","offer_id":42882222981299,"sku":"PR3010-10","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_7c650895-3deb-4a55-aa81-982d8a518e9b.jpg?v=1702049554","url":"https:\/\/afsbio.com\/products\/recombinant-rat-interleukin-21-rrtil-21-pr3010","provider":"AFSBio Inc.","version":"1.0","type":"link"}