{"product_id":"recombinant-rat-rb1-protein-his-tag-pder100076","title":"Recombinant Rat RB1 protein (His tag) - PDER100076","description":"\u003cp\u003eRecombinant Rat RB1 protein (His tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PDER100076-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: RB1\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: OSRC; osteosarcoma; p105-Rb; pp110; pRb; RB; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; retinoblastoma-associated protein\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P33568\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Epigenetics and Nuclear Signaling; Developmental Biology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Rat\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: N-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: P33568\u003c\/p\u003e\n\n\u003cp\u003eBackground: Retinoblastoma 1 protein (RB-1; also retinoblastoma-associated protein, pp110, and p105-Rb) is a 110 kDa tumor suppressor gene and member of the retinoblastoma protein family. Rat RB-1 is 920 amino acids in length. The protein contains a Pocket domain (aa 366-763), which is comprised of two other domains, domain A (aa 366-572) and domain B (aa 632-763), and a “spacer” (aa 573-631). The Pocket domain binds to threonine-phosphorylated domain C (aa 763-920), which thereby prevents interaction with heterodimeric E2F\/DP transcription factor complexes. RB-1 is expressed in the retina. The underphosphorylated, active form of RB-1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Defects in RB-1 lead to the childhood cancer retinoblastoma.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Arg 229-Met 450\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 24.31 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 32 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43083605213363,"sku":"PDER100076-100","price":165.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_7dd8ac32-3ca6-4cff-91f4-e546844a776d.png?v=1708945092","url":"https:\/\/afsbio.com\/products\/recombinant-rat-rb1-protein-his-tag-pder100076","provider":"AFSBio Inc.","version":"1.0","type":"link"}