{"product_id":"recombinant-rat-tnf-alpha-tnfa-protein-active-pksr030443","title":"Recombinant Rat TNF-alpha\/TNFA Protein (Active) - PKSR030443","description":"\u003cp\u003eRecombinant Rat TNF-alpha\/TNFA Protein (Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 20μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSR030443-20\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: TNF-alpha; TNFA\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Tnf; MGC124630; RATTNF; TNF-alpha; Tnfa\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Rat\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P16599\u003c\/p\u003e\n\n\u003cp\u003eAccession: P16599\u003c\/p\u003e\n\n\u003cp\u003eBackground: Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55\/60) and TNF-R2 (TNF receptor type 2; CD120b; p75\/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 20-80 pg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Leu 80-Leu 235\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 93 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 17.4 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 15 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43107248996531,"sku":"PKSR030443-20","price":393.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_de70e257-d08a-496c-acdf-a43c9bec674f.png?v=1709381921","url":"https:\/\/afsbio.com\/products\/recombinant-rat-tnf-alpha-tnfa-protein-active-pksr030443","provider":"AFSBio Inc.","version":"1.0","type":"link"}