{"product_id":"recombinant-rat-trkb-ntrk2-protein-his-tagactive-pksr030292","title":"Recombinant Rat TrkB\/NTRK2 Protein (His Tag)(Active) - PKSR030292","description":"\u003cp\u003eRecombinant Rat TrkB\/NTRK2 Protein (His Tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSize: 20μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSR030292-20\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: TrkB; NTRK2\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: NTRK2; Trkb\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Rat\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q63604\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q63604\u003c\/p\u003e\n\n\u003cp\u003eBackground: TrkB receptor also known as TrkB tyrosine kinase or BDNF\/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB\/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42\/Rac\/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB\/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB\/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB\/NTRK have been associated with obesity and mood disorders.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Immobilized rat NTRK2-His at 10 μg\/ml (100 μl\/well) can bind biotinylated mouse BDNF, The EC50 of biotinylated mouse BDNF is 12.2-28.6 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met1-His429\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 47.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 67 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"Default Title","offer_id":43104369279155,"sku":"PKSR030292-20","price":541.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_d4dfd75a-06dc-4310-bb1f-f0288c5ef889.png?v=1709295349","url":"https:\/\/afsbio.com\/products\/recombinant-rat-trkb-ntrk2-protein-his-tagactive-pksr030292","provider":"AFSBio Inc.","version":"1.0","type":"link"}