{"product_id":"recombinant-rat-tumor-necrosis-factor-alpha-rrttnf-α-pr3014","title":"Recombinant Rat Tumor Necrosis Factor-alpha (rRtTNF-α) - PR3014","description":"\u003cp\u003eRecombinant Rat Tumor Necrosis Factor-alpha (rRtTNF-α)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PR3014-5, PR3014-20\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5µg, 20µg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: Approximately 17.3 kDa. a single, non-glycosylated polypeptide chain containing 157 amino acids.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt;95% by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Fully biologically active when compared to standard. The Specific Activity is ≥5.0 × 107 IU\/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH7.2, 150mM NaCl.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MLRSSSQNSS DKPVVHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV\u003cbr\u003e\n VPADGLYLIY SQVLFKGQGC PDYVLLTHt V SRFATSYQEK VSLLSAIKSP\u003cbr\u003e\n CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV\u003cbr\u003e\n YFGVIAL\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 1EU\/mg of rRtTNF-α as determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/ml. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China\u003c\/p\u003e\n\n\u003cp\u003eDescription: Tumor necrosis factor alpha (TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"5µg","offer_id":42882230550707,"sku":"PR3014-5","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"20µg","offer_id":42882230583475,"sku":"PR3014-20","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_18cbd264-3998-444a-b959-eb253bda0beb.jpg?v=1702049718","url":"https:\/\/afsbio.com\/products\/recombinant-rat-tumor-necrosis-factor-alpha-rrttnf-%ce%b1-pr3014","provider":"AFSBio Inc.","version":"1.0","type":"link"}