{"product_id":"recombinant-s-cerevisiae-tim14-protein-pksq050085","title":"Recombinant S. cerevisiae TIM14 Protein - PKSQ050085","description":"\u003cp\u003eRecombinant S. cerevisiae TIM14 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSQ050085-10, PKSQ050085-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: TIM14\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Mitochondrial import inner membrane translocase subunit TIM14; Presequencetranslocated-associated motor subunit PAM18; PAM18; TIM14\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q07914\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: S. cerevisiae\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q07914\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mitochondrial import inner membrane translocase subunit TIM14 (TIM14) is an essential component of the PAM complex. PAM complex is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, TIM14 is required to stimulate activity of mtHSP70 (SSC1). TIM14 belongs to the DnaJ family, which has been involved in Hsp40\/Hsp70 chaperone systems. As a mitochondrial chaperone, TIM14 functions as part of the TIM23 complex import motor to facilitate the import of nuclear-encoded proteins into the mitochondria. TIM14 also complexes with prohibitin complexes to regulate mitochondrial morphogenesis, and has been implicated in dilated cardiomyopathy with ataxia.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Phe99-Lys168\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, pH8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 7.9 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 9 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43072231243955,"sku":"PKSQ050085-10","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43072231276723,"sku":"PKSQ050085-50","price":732.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_b5331996-9552-47b5-bd3d-f658ee0915cf.png?v=1708684765","url":"https:\/\/afsbio.com\/products\/recombinant-s-cerevisiae-tim14-protein-pksq050085","provider":"AFSBio Inc.","version":"1.0","type":"link"}