{"product_id":"recombinant-s-cerevisiae-tim16-protein-pksq050084","title":"Recombinant S. cerevisiae TIM16 Protein - PKSQ050084","description":"\u003cp\u003eRecombinant S. cerevisiae TIM16 Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSQ050084-10, PKSQ050084-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: TIM16\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Mitochondrial import inner membrane translocase subunit TIM16; Presequence translocated-associated motor subunit PAM16; PAM16; TIM16\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P42949\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: S. cerevisiae\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eAccession: P42949\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mitochondrial import inner membrane translocase subunit TIM16 (TIM16) is an ssential component of the PAM complex. PAM complex is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, TIM16 is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18\/TIM14. TIM16 may act by positioning PAM18\/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Thr54-Ala119\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, pH8.0.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 7.9 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 11 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43072231112883,"sku":"PKSQ050084-10","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43072231145651,"sku":"PKSQ050084-50","price":732.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_5a5b3362-0e61-4847-9615-20b9e8c4506d.png?v=1708684760","url":"https:\/\/afsbio.com\/products\/recombinant-s-cerevisiae-tim16-protein-pksq050084","provider":"AFSBio Inc.","version":"1.0","type":"link"}