{"product_id":"recombinant-sars-cov-2-2019-ncov-s-protein-rbd-c-mfc-6his-tagactive-pksv030276","title":"Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD (C-mFc-6His tag)(Active) - PKSV030276","description":"\u003cp\u003eRecombinant SARS-CoV-2 (2019-nCoV) S protein RBD (C-mFc-6His tag)(Active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSV030276-50, PKSV030276-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Spike\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: S protein RBD; Spike glycoprotein Receptor-binding domain; S glycoprotein RBD; Spike protein RBD; COVID-19\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: SARS-CoV-2\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-mFc-His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P0DTC2\u003c\/p\u003e\n\n\u003cp\u003eAccession: QHD43416.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.\u003c\/p\u003e\n\n\u003cp\u003eActivity: 1. ELISA plate pre-coated by 2 μg\/ml (100 μl\/well) Human S-RBD, mFc-His tagged protein (PKSV030276) can bind Anti-SARS-CoV-2 Neutralizing antibody(E-AB-V1028) in a linear range of 0.24-15.62 ng\/ml. \u003cbr\u003e\n2. ELISA plate pre-coated by 2 μg\/ml (100 μl\/well) S-RBD, mFc-His tagged protein (PKSV030276) can bind Human ACE2, hFc Tagged protein（PKSR030492） in a linear range of 0.488-49.83 ng\/ml.\u003c\/p\u003e \n\n\u003cp\u003eSequence: Arg319-Phe541\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 52.2 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"50μg","offer_id":43118190428339,"sku":"PKSV030276-50","price":910.0,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":43118190461107,"sku":"PKSV030276-100","price":1365.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_c6617286-a301-48f7-89fc-020a647dc581.png?v=1709637806","url":"https:\/\/afsbio.com\/products\/recombinant-sars-cov-2-2019-ncov-s-protein-rbd-c-mfc-6his-tagactive-pksv030276","provider":"AFSBio Inc.","version":"1.0","type":"link"}