{"product_id":"recombinant-sars-cov-spike-rbd-protein-rp01304","title":"Recombinant SARS-CoV Spike RBD Protein - RP01304","description":"\u003cp\u003eRecombinant SARS-CoV Spike RBD Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10ug, 100ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: RP01304-10, RP01304-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant SARS-CoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg306-Phe527) of sars-cov Spike RBD (Accession #NP_828851.1) fused with mFc tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Spike, Spike RBD, Spike S1\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P59594\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 1489668\u003c\/p\u003e\n\n\u003cp\u003eSpecies: SARS-CoV\u003c\/p\u003e\n\n\u003cp\u003ePurity: ≥ 95 % as determined by SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\n\u003cp\u003eBackground: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.\u003c\/p\u003e\n\n\u003cp\u003eBio-Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV Spike RBD at 2μg\/mL (100μL\/well) can bind Human ACE2 (Catalog: RP01266) with a linear range of 0.1-11.56 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK293 cells\u003c\/p\u003e\n\n\u003cp\u003eTag: C-mFc\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003eAntigen Sequence: RVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNF\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.1 EU\/μg of the protein by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"10ug","offer_id":45198358675635,"sku":"RP01304-10","price":196.0,"currency_code":"CAD","in_stock":true},{"title":"100ug","offer_id":45198358708403,"sku":"RP01304-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_a9b89901-efb1-4982-b1c2-44f41b699e02.png?v=1766961121","url":"https:\/\/afsbio.com\/products\/recombinant-sars-cov-spike-rbd-protein-rp01304","provider":"AFSBio Inc.","version":"1.0","type":"link"}