{"product_id":"recombinant-streptomyces-hygroscopicus-bar-protein-pksq050087","title":"Recombinant Streptomyces hygroscopicus Bar Protein - PKSQ050087","description":"\u003cp\u003eRecombinant Streptomyces hygroscopicus Bar Protein\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSQ050087-10, PKSQ050087-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Bar\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; Phosphinothricin-resistance protein; bar\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P16426\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Streptomyces hygroscopicus\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: None\u003c\/p\u003e\n\n\u003cp\u003eAccession: P16426\u003c\/p\u003e\n\n\u003cp\u003eBackground: Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met1-Ile183\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 20.6 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 18-20 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43072231473331,"sku":"PKSQ050087-10","price":180.6,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43072231506099,"sku":"PKSQ050087-50","price":525.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_76d44bc7-53d9-485e-b131-7735575ac696.png?v=1708684770","url":"https:\/\/afsbio.com\/products\/recombinant-streptomyces-hygroscopicus-bar-protein-pksq050087","provider":"AFSBio Inc.","version":"1.0","type":"link"}