{"product_id":"recombinant-swine-il-8-proteinn-hisactive-pkss000006","title":"Recombinant Swine IL-8 protein(N-His)(active) - PKSS000006","description":"\u003cp\u003eRecombinant Swine IL-8 protein(N-His)(active)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 5μg, 20μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSS000006-5, PKSS000006-20\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: IL-8\u003c\/p\u003e\n\n\u003cp\u003eTarget Symptom: Monocyte-Derived Neutrophil Chemotactic Factor (MDNCF); Neutrophil Activating Factor (NAF); AMCF-I; CXCL8\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Porcine\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eApplication: Cell culture\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P26894\u003c\/p\u003e\n\n\u003cp\u003eAccession: P26894\u003c\/p\u003e\n\n\u003cp\u003eBackground: Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte\/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia\/anoxia may stimulate expression. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8\/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental chang. Numerous observations have established IL-8\/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. The discovery of these biological functions suggests that IL-8\/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer.\u003c\/p\u003e\n\n\u003cp\u003eActivity: Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2. The ED50 for this effect is \u0026lt; 5 ng\/mL.\u003c\/p\u003e\n\n\u003cp\u003eSequence: MTSKLAVAFLAVFLLSAALCEAAVLARVSAELRCQCINTHSTPFHPKFIKELRVIESGPHCENSEIIVKLVNGKEVCLDPKEKWVQKVVQIFLKRTEKQQQQQ\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 98 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Please contact us for more information.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 12.46 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"5μg","offer_id":43118188593331,"sku":"PKSS000006-5","price":277.2,"currency_code":"CAD","in_stock":true},{"title":"20μg","offer_id":43118188626099,"sku":"PKSS000006-20","price":693.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_6840aacc-f01d-4af8-a483-36b54b4a4d32.png?v=1709637764","url":"https:\/\/afsbio.com\/products\/recombinant-swine-il-8-proteinn-hisactive-pkss000006","provider":"AFSBio Inc.","version":"1.0","type":"link"}