{"product_id":"recombinant-tev-protease-his-bk0165","title":"Recombinant TEV Protease, His - BK0165","description":"\u003cp\u003eRecombinant TEV Protease, His\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0165-1000, BK0168-10\u003c\/p\u003e\n\n\u003cp\u003eSizes: 1000IU, 10μg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 28.4 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% by SDS-PAGE analyses.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: 6 IU\/μl. Unit Definition: One unit of TEV protease cleaves \u0026gt; 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH7.5.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: 0\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin:\u003c\/p\u003e \n\n\u003cp\u003eReconstitution:\u003c\/p\u003e \n\n\u003cp\u003eStorage: Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly\/Ser) [ENLYFQ(G\/S)] and cleavage occurs between the Gln and Gly\/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"1000IU","offer_id":42882246869171,"sku":"BK0165-1000","price":444.71,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d157edeb-cd1d-46aa-a262-f49832977413.jpg?v=1702050009","url":"https:\/\/afsbio.com\/products\/recombinant-tev-protease-his-bk0165","provider":"AFSBio Inc.","version":"1.0","type":"link"}