{"product_id":"recombinant-tsg-his-human-bk0334","title":"Recombinant TSG, His, Human - BK0334","description":"\u003cp\u003eRecombinant TSG, His, Human\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0334-10, BK0334-50\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eSource: HEK 293\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 30-33 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% as analyzed by SDS-PAGE and HPLC.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Determined by its ability to neutralize BMP-6 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is \u0026lt; 2 μg\/ml of TSG.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against PBS.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: HHHHHHHHCNKALCASDVSKCLIQELCQCRPGEGNCSCCKECMLCLGALWDECCDCVGMCNPRNYSDT\u003cbr\u003e\n PPTSKSt VEELHEPIPSLFRALTEGDTQLNWNIVSFPVAEELSHHENLVSFLEt VNQPHH\u003cbr\u003e\n QNVSVPSNNVHAPYSSDKEHMCt VVYFDDCMSIHQCKISCESMGASKYRWFHNACCECIG\u003cbr\u003e\n PECIDYGSKt VKCMNCMF\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in ddH2O or PBS at 100 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant Human Twisted Gastrulation (TSG), remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human Twisted Gastrulation should be stable up to 1 week at 4°C or up to 2 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Twisted gastrulation (TWSG1 or TSG) is a cysteine-rich 24 kDa glycoprotein. It is a secreted BMP binding protein that modulates BMP ligand availability in extracellular space. Human TSG shares 98% aa identity with mouse and rat TSG, and 99.5% aa identity with canine, equine, bovine and porcine TSG. Glycosylation and bioactivity of TWSG1 recombinant proteins vary markedly by cellular source. Non-glycosylated hTWSG1 made in E. coli has both reduced affinity for BMPs, as shown by surface plasmon resonance analysis, and reduced BMP inhibitory activity in a mandibular explant culture system compared to glycosylated proteins made in insect cells or mouse myeloma cells.Recombinant human Twisted Gastrulation (TSG), produced in HEK 293 cells is a polypeptide chain containing 211 amino acids. A fully biologically active molecule, rhTSG has a molecular mass of 30~33 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"10μg","offer_id":42882263974067,"sku":"BK0334-10","price":217.41,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":42882264006835,"sku":"BK0334-50","price":691.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_16d451d6-a079-405b-8aed-7ef653abed5f.jpg?v=1702050332","url":"https:\/\/afsbio.com\/products\/recombinant-tsg-his-human-bk0334","provider":"AFSBio Inc.","version":"1.0","type":"link"}