{"product_id":"recombinant-vegf164-mouse-p-pastoris-expre-ssed-bk0350","title":"Recombinant VEGF164, Mouse (P.pastoris-expre Ssed) - BK0350","description":"\u003cp\u003eRecombinant VEGF164, Mouse (P.pastoris-expre Ssed)\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BK0350-20, BK0350-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μg, 100μg\u003c\/p\u003e\n\n\u003cp\u003eSource: P. pastoris\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 39kDa, observed by non-reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 97% as analyzed by reducing SDS-PAGE\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Resuspend cells in assay media (Earle Salts 199X Gibco #11150, without h-EGF) containing 10 % HI-FBS and transfer 100 ul\/well to assay plate (5,100 cells\/well passage#2). Serial dilute murine VEGF in assay media containing 10 % HI-FBS and transfer 100ml\/well to cells in assay plate. Final assay volume is 200 ml\/well, containing 10 % HI-FBS, and murine VEGF as indicated 89 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well, Incubate 37°C and read at OD 490 nm. The ED50 for the effect is typically 4.1 - 6.2 ng\/ml.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: MAPTTE GEQKSHEVIK FMDVYQRSYC RPIETLVDIF QEYPDEIEYI FKPSCVPLMR CAGCCNDEAL ECVPTSESNI TMQIMRIKPH QSQHIGEMSF LQHSRCECRP KKDRTKPEKH CEPCSERRKH LFVQDPQTCK CSCKNTDSRC KARQLELNER TCRCDKPRR\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt;1.0 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in ddH2O at 100 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant mouse Vascular Endothelial Growth Factor A164 (rmVEGF-A164)remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rmVEGF-A164 should be stable up to 4 week at 4°C or up to 6 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant mouse Vascular Endothelial Growth Factor A164 (rmVEGF-A164) produced in Pichia pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rmVEGF-A164 has a molecular mass of 39kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"20μg","offer_id":42882265841843,"sku":"BK0350-20","price":375.54,"currency_code":"CAD","in_stock":true},{"title":"100μg","offer_id":42882265874611,"sku":"BK0350-100","price":1152.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_0fda99a0-2b5b-4755-b1e0-70b2faaf384d.jpg?v=1702050369","url":"https:\/\/afsbio.com\/products\/recombinant-vegf164-mouse-p-pastoris-expre-ssed-bk0350","provider":"AFSBio Inc.","version":"1.0","type":"link"}