{"product_id":"rev-erbα-polyclonal-antibody-gcp108","title":"Rev-Erbα Polyclonal Antibody-GCP108","description":"\u003cp\u003eRev-Erbα Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: GCP108-50, GCP108-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P20393\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human,Pig\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:5000~1:10000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins. Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells. In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm. Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK-3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock. Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2. Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)\/HDAC3 complex to target genes to repress transcription.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Rev-Erbα polyclonal antibody detects endogenous levels of Rev-Erbα protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Rev-Erbα polyclonal antibody at 1:10000 dilution\u003cbr\u003e\nLane1:U-87MG whole cell lysate(40ug)\u003cbr\u003e\nLane2:A549 whole cell lysate(40ug)\u003cbr\u003e\nLane3:The Lung tissue lysate of Pig(40ug)\u003cbr\u003e\nLane4:HepG2 whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 78 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1; EAR-1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human Rev-Erbα.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42908756115635,"sku":"GCP108-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42908756148403,"sku":"GCP108-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a19e357c-cdce-4772-9079-5a57ddf396cb.jpg?v=1703006694","url":"https:\/\/afsbio.com\/products\/rev-erb%ce%b1-polyclonal-antibody-gcp108","provider":"AFSBio Inc.","version":"1.0","type":"link"}