
Ro RNP
SSA antigenRo60 antigen
Sjögren's syndrome antigen A
Source: Calf Thymus
Catalogue Number: ATR02-02
Product Information
The incidence of anti-Ro (SSA) antibodies in the population of normal women of reproductive age is in the order of 1%. The presence of anti-La (SSB) autoantibodies usually coincides with the presence of anti-Ro (SSA).
Ro(SSA) antibodies target protein antigens associated with small RNA molecules known as hY-RNAs. These protein-RNA complexes are referred to as Ro-ribonucleoproteins (Ro-RNPs) and their biological function has yet to be elucidated. It is generally acknowledged that a 60kDa protein (Ro60) intimately associated with Ro-RNP constitutes the major Ro(SSA) antigen. The binding of autoantibodies to Ro60 is heterogenous: some sera recognise both the native and denatured antigen while others recognise the native antigen conformation only. The association of a second putative Ro(SSA) antigen of 52kDa (Ro52) with Ro-RNP remains unclear.
Although it has been proposed that Ro52 may be indirectly associated with Ro-RNP through Ro60, it has been conversely demonstrated that Ro52 does not copurify with Ro-RNPs during chromatographic separation. Furthermore true monospecific anti-Ro52 sera are known to be rare, they do not display a typical Ro(SSA) immunofluorescence pattern and are negative in immunodiffusion.
Clinical Indications
Sjögren's syndrome
Systemic Lupus Erythematosus
Rheumatoid Arthritis
Neonatal Lupus Syndrome
MSDS
References
Tan, E.M. (1989) Adv. Immunol. 44, 93
McCauliffe, D.P. & Sontheimer, R.D. (1993) J. Inv. Dermatol. 100, 73
Harley, J.B. et al. (1986) Arthritis Rheum. 29, 196
Reichlin, M. (1986) J. Clin. Immunol. 6, 339
Boire, G. et al. (1993) J. Rheumatol. 20, 1654
Harley, J.B. et al. (1992) Rheum. Dis. Clin. N. America 18, 337
Calmes, M. & Bartholomew, B.A. (1985) J. Clin. Pathol. 38, 73
Taylor, P.V. et al. (1988) Br. J. Rheumatol. 27, 128
Craft, J.E. & Hardin, J.A. (1987) J. Rheumatol. 14 S13, 106
St. Clair, E.W. (1992) Rheum. Dis. Clin. N. America 18, 359
Hendrick, J.P. et al. (1981) Mol. Cell Biol. 1, 1138
Wolin, S.L. et al. (1984) 81, 1996
Chan, E.K.L. & Buyon, J.P. (1994) Man. Biol. Markers Dis.(Kluwer Acad. Publ.) B1/1-18
Boire, G. et al. (1991) Arthritis Rheum. 34, 722
Itoh, Y. & Reichlin, M. (1992) Autoimmunity 14, 57
Tsuzaka, K. et al. (1994) Arthritis Rheum. 37, 88
Slobbe, R.L. et al. (1992) J. Mol. Biol. 227, 361
Boire, G. et al. (1995) Clin. Exp. Immunol. 100, 489
Meheus, L. et al. (1998) 4th Dresden Symp. Autoantibodies (Abstract)
Deutscher, S.L. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 9479
Ben-Chetrit, E. et al. (1989) J. Clin. Invest. 83, 1284
Wang, D. et al. (1996) Mol. Biol. Rep. 23, 205
Gaither, K.K. & Harley, J.B. (1985) Prot. Biol. Fluids Proc.Coll. 33, 413
Yamagata, H. et al. (1984) J. Clin. Invest. 74, 625
Yoshida, S. & Reichlin, M. (1990) J. Immunol. Methods 131, 113