{"product_id":"rpa1-monoclonal-antibody-mb0164","title":"RPA1 Monoclonal Antibody-MB0164","description":"\u003cp\u003eRPA1 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB0164-50, MB0164-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P27694\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat, Monkey\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,  ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000 ICC: 1:100~300 IP: 1:50~100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Plays an essential role in several cellular processes in DNA metabolism including replication,recombination and DNA repair.Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA).aRPA binds single-stranded DNA and probably plays a role in DNA repair;it does not support chromosomal DNA replication and cell cycle progression through S-phase.In vitro,aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC),the dual incision\/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of RPA1 and does not cross-react with related proteins\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Predicted band size: 70KDa\u003cbr\u003e\nObserved band size: 70KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot detection of RPA1 in HUVEC,Hacat,3T3,MCF7 and A549 cell lysates using RPA1 mouse mAb (1:2000 diluted).., Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA1 mouse mAb (dilution 1:100)., Immunoprecipitation analysis of Hela cell lysates using RPA1 mouse mAb.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Dmrpa1; Drosophila Replication Protein A; DRPA; HSSB; Human single stranded DNA binding protein; MST075; MSTP075; p70; REPA1; Replication factor A; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; Replication protein A 70kDa DNA binding subunit; Replication protein A1 70kDa; Replication protein A1; RF A; RF-A protein 1; RFA; RFA1; RP A; RP-A p70; RPA 70; RPA; RPA1; Single stranded binding protein 70; Single-stranded DNA-binding protein\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant full length Human RPA1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42997589147827,"sku":"MB0164-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42997589180595,"sku":"MB0164-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_931bb903-0a78-42d4-8c61-0cd7ec5807b2.jpg?v=1706270792","url":"https:\/\/afsbio.com\/products\/rpa1-monoclonal-antibody-mb0164","provider":"AFSBio Inc.","version":"1.0","type":"link"}