{"product_id":"rpb1-phospho-s1619-polyclonal-antibody-bs4246","title":"Rpb1 (phospho-S1619) Polyclonal Antibody-BS4246","description":"\u003cp\u003eRpb1 (phospho-S1619) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4246-50, BS4246-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P24928\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eRpb1 (phospho-S1619) Polyclonal Antibody-BS4246\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-Rpb1 (S1619) polyclonal antibody detects endogenous levels of Rpb1 protein only when phosphorylated at Ser1619.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 280 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-Rpb1 (S1619) pAb at 1:2000 dilution\u003cbr\u003e\n Lane 1: A549 whole cell lysate (40ug) \u003cbr\u003e\n Lane 2: Hela whole cell lysate (40ug) \u003cbr\u003e\n Lane 3: A2780 whole cell lysate (40ug) \u003cbr\u003e\n Lane 4: PC12 whole cell lysate (40ug) \u003cbr\u003e\n Lane 5: 3T3-L1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Rpb1 (S1619) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2A; POLR2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human Rpb1 around the phosphorylation site of Serine 1619.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957297942707,"sku":"BS4246-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957297975475,"sku":"BS4246-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_9dac7e46-90e3-44f9-b1ba-96f4c9362b1a.jpg?v=1705144522","url":"https:\/\/afsbio.com\/products\/rpb1-phospho-s1619-polyclonal-antibody-bs4246","provider":"AFSBio Inc.","version":"1.0","type":"link"}