{"product_id":"rps6ka4-polyclonal-antibody-bs74379","title":"RPS6KA4 Polyclonal Antibody-BS74379","description":"\u003cp\u003eRPS6KA4 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74379-50, BS74379-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O75676\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eRPS6KA4 Polyclonal Antibody-BS74379\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:200 - 1:1000  |  IHC,1:20 - 1:100  |  IF\/ICC,1:20 - 1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine\/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 85KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of U-87MG cells, using RPS6KA4 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 180s., Immunohistochemistry of paraffin-embedded mouse lung using RPS6KA4 antibody at dilution of 1:200.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: RPS6KA4; MSK2; RSK-B; S6K-alpha-4\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human RPS6KA4(NP_003933.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957339295923,"sku":"BS74379-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957339328691,"sku":"BS74379-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e1a9cdd3-8ec3-4689-9ef8-201d45f0d407.jpg?v=1705145062","url":"https:\/\/afsbio.com\/products\/rps6ka4-polyclonal-antibody-bs74379","provider":"AFSBio Inc.","version":"1.0","type":"link"}