{"product_id":"sarm1-polyclonal-antibody-bs78063","title":"SARM1 Polyclonal Antibody-BS78063","description":"\u003cp\u003eSARM1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS78063-50, BS78063-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q6SZW1\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eSARM1 Polyclonal Antibody-BS78063\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000  |  IP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: NAD(+ hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+ metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+ depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+ into ADP-D-ribose (ADPR, cyclic ADPR (cADPR and nicotinamide; NAD(+ cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+, but not other NAD(+-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway (By similarity. Involved in innate immune response: inhibits both TICAM1\/TRIF- and MYD88-dependent activation of JUN\/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14\/p38.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 73KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using SARM1 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 90s., Immunoprecipitation analysis of 300ug extracts of SH-SY5Y cells using 3ug SARM1 antibody. Western blot was performed from the immunoprecipitate using SARM1 antibody at a dilution of 1:1000.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: SARM; HsTIR; SAMD2; hSARM1; MyD88-5\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human SARM1(NP_055892.2).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42960083386547,"sku":"BS78063-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42960083452083,"sku":"BS78063-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d27b194f-e792-4774-aba3-674664ea171c.jpg?v=1705231327","url":"https:\/\/afsbio.com\/products\/sarm1-polyclonal-antibody-bs78063","provider":"AFSBio Inc.","version":"1.0","type":"link"}